Coding

Part:BBa_K3619007:Design

Designed by: Isak Ingerholt   Group: iGEM20_Lund   (2020-09-13)


cDNA encoding the anti microbial peptide Ec-AMP-D1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon optimized for expression in E. coli K12.


Source

The peptide originates from Echinochloa crusgalli, the annual weed barnyard grass. DNA sequence was obtained through back translation.

References

1. https://pubmed.ncbi.nlm.nih.gov/18625284/

2. https://www.uniprot.org/uniprot/P86518